Antifungal Activity of Clove against Phytopathogenic Fungi and HPLC analysis of Extract

The objectives of this work were to evaluate the antifungal activity of clove flower buds extracts in management of three pathogenic fungus were isolated from strawberry, which molecularly identified through Internal Transcribed Spacer (ITS) ribosomal RNA (rRNA) sequencing, Alternaria alternata (accession no. PP737870), Botrytis cinerea (accession no. PP758474) and Fusarium oxysporum (accession no. PP737874). The results of study revealed weak to high level antifungal activities against tested phytopathogenic fungi at concentrations of 2000, 1000, 500 and 250 ?l ml - 1. The highest antifungal activity was recorded at 2000 ?Lml - 1; on this concentration clove flower buds extract caused growth inhibition of A. alternata, B. cinerea, F. oxysporum were observed 50.00%, 60.37%, 76.30% respectively, while the weak antifungal activity against A. alternata, B. cinerea, F. oxysporum at 500 ?Lml - 1 were observed 8.15%, 5.93%, 28.89% respectively. The inhibition rate of the growth of the three fungi with the pesticide (Topsin - M 70 WP) was 75.56 to 100% at 2 ?l/ml concentration, compare to the inhibition rate with the control was 0.00%. Clove flower buds extract was analyzed using High Performance Liquid Chromatography (HPLC) for phenolic and flavonoid compounds, the highest amounts of polyphenolic compounds viz., Ferulic acid, P - Coumaric, Caffeic acid, Catechol, Syringenic acid, Gallic acid and Cinnamic with 13.95, 13.68, 13.52, 7.31, 6.82, 6.65and 2.20 ?g/gm, respectively. Application of readily available aqueous plant extracts to controlling plant disease pathogens is an eco - friendly.