Pickering Emulsion Stabilized by Different Concentrations of Whey Protein–Cress Seed Gum Nanoparticles

Nanoparticles based on food-grade materials are promising materials to develop Pickering emulsions for food applications. Initially, this study focuses on the development of nanoparticles through the utilization of a soluble complex of whey protein concentrate (WPC) and cress seed gum (CSG), which were modified by calcium chloride (CaCl₂) as a cross-linker. The response surface methodology was used to investigate the impact of different concentrations of WPC (1-4% <i>w</i>/<i>v</i>), CSG (0-1% <i>w</i>/<i>v</i>), and CaCl₂ (1-3 mM) on particle size, polydispersity index (PDI), and Zeta potential. The optimum conditions for the production of CSG-WPC nanoparticles (WPC-CSG NPs) were 0.31% (<i>w</i>/<i>v</i>) CSG, 1.75% (<i>w</i>/<i>v</i>) WPC, and 1.69 mM CaCl₂, resulting in nanoparticles with average size of 236 nm and Zeta potential of -22 mV. Subsequently, oil-in-water (O/W) Pickering emulsions were produced with different concentrations of WPC-CSG NPs in optimum conditions. The contact angles of the WPC-CSG NPs were 41.44° and 61.13° at concentrations of 0.5% and 1%, respectively, showing that NPs are suitable for stabilizing O/W Pickering emulsions. Pickering emulsion viscosity rose from 80 to 500 mPa when nanoparticle concentration increased from 0.5% to 1%. Results also showed that WPC-CSG NPs enable stable O/W Pickering emulsions during storage and thermal treatment, confirming that protein-polysaccharide NPs can provide a sufficient steric hindrance.