Easy, Rapid and High‐Throughput Production of Single and Multicompartment Liposomes by Vortex Emulsification

Abstract Cell‐sized liposomes, the self‐assembled phospholipid vesicles with bilayer membranes, have garnered substantial attention across various fields, particularly as cell mimics. In this study, we introduce a simplified method for rapidly producing single‐ and multicompartment liposomes using a common laboratory vortex mixer. The simplicity of our method has the potential to greatly facilitate artificial cell and tissue‐related research, potentially leading to broader applications of liposomes.