Stochastic trade-offs and the emergence of diversification in <i>E. coli</i> evolution experiments

Laboratory experiments with bacterial colonies under well-controlled conditions often lead to evolutionary diversification, where at least two ecotypes emerge from an initially monomorphic population. Empirical evidence suggests that such an “evolutionary branching” occurs stochastically, even under fixed and stable conditions. This stochasticity is characterized by (i) the occurrence of branching in a significant fraction, but not all, of the experimental settings, (ii) the emergence at widely varying times, and (iii) variable relative abundances of the resulting subpopulations across experiments. Theoretical approaches to understanding evolutionary branching under these conditions have been previously developed within the (deterministic) framework of “adaptive dynamics.” Here, we advance the understanding of the stochastic nature of evolutionary outcomes by introducing the concept of “stochastic trade-offs” as opposed to “hard” ones. The key idea is that the stochasticity of mutations occurs in a high-dimensional trait space and this translates into variability that is constrained to a flexible tradeoff curve in a lower-dimensional space. By incorporating this additional source of stochasticity, we are able to account for the observed empirical variability and make predictions regarding the likelihood of evolutionary branching under different experimental conditions. This approach effectively bridges the gap between theoretical predictions and empirical observations, providing insights into when and how evolutionary branching is more likely to occur in laboratory experiments. Published by the American Physical Society 2024