Flow Cytometry Analyses of Meningioma Immune Cell Composition Using a Short, Optimized Digestion Protocol
We successfully optimized a protocol for generating single-cell suspensions with viable immune cells from meningioma tissues, revealing infiltrating antigen-presenting cells with an immunosuppressive phenotype, and lymphocytes. This short protocol allows advanced analyses of tumor-infiltrating cells using techniques such as single-cell RNA sequencing and flow cytometry, which require live, dissociated cells.
Authors
- Tiit Illimar Mathiesen (ORCID: https://orcid.org/0000-0001-9463-1919)
- Jeppe Lohfert Haslund-Vinding (ORCID: https://orcid.org/0000-0003-2941-3573)
- Janusz Lange
- Josephine Bjørn Budde (ORCID: https://orcid.org/0000-0001-8119-8036)
- Frederik Vilhardt (ORCID: https://orcid.org/0000-0001-8604-7165)
- Anders Woetmann (ORCID: https://orcid.org/0000-0002-3008-735X)
- Nadja Blum (ORCID: https://orcid.org/0009-0009-7722-7952)
- Lars Ohlsson (ORCID: https://orcid.org/0000-0002-4044-5923)
- Gillian Dao Nyesiga
- Kotryna Dukstaite
Institutions
- University of Copenhagen (DK)
- Malmö University (SE)
- Copenhagen University Hospital (DK)
- Rigshospitalet (DK)
- Karolinska Institutet (SE)
- LEO Foundation (DK)
Publication Details
- Journal
- Cancers
- Published
- 2024-11-25
- DOI
- https://doi.org/10.3390/cancers16233942
- Citations
- 1
- Primary Topic
- Meningioma and schwannoma management
- Type
- article
- Field-Weighted Citation Impact
- 0.39
Funders
- LEO Fondet
- H. Lundbeck A/S