Establishment of a Real-Time Reverse Transcription Recombinase-Aided Isothermal Amplification (qRT-RAA) Assay for the Rapid Detection of Bovine Respiratory Syncytial Virus
A qRT-RAA assay for BRSV detection was established in this study. The method is specific and sensitive and can be completed within 20 min at 39 °C. These works demonstrate that the generated qRT-RAA assay is an effective diagnostic tool for rapidly detecting BRSV in resource-limited settings, which may be applied for the clinical detection of BRSV.
Authors
- Chihuan Li
- Xintong Zhu
- Qiumei Shi (ORCID: https://orcid.org/0000-0002-4882-6309)
- Zhiqiang Zhang (ORCID: https://orcid.org/0000-0003-1796-081X)
- Hong Li (ORCID: https://orcid.org/0000-0001-6930-4772)
- Xiaochen Liu (ORCID: https://orcid.org/0000-0001-9824-094X)
- Siping Zhu
- Chao Ren
- Guanxin Hou
Institutions
- Hebei Normal University of Science and Technology (CN)
Publication Details
- Journal
- Veterinary Sciences
- Published
- 2024-11-24
- DOI
- https://doi.org/10.3390/vetsci11120589
- Citations
- 4
- Primary Topic
- Respiratory viral infections research
- Type
- article
- Field-Weighted Citation Impact
- 1.55