Resolving Spatiotemporal Electrical Signaling Within the Islet via CMOS Microelectrode Arrays
The high-resolution complementary metal-oxide-semiconductor multielectrode array is suited to track the spatiotemporal propagation of electrical activity through the islet on a cellular scale. A highly responsive subpopulation of islet cells was identified by action potential-like spike activity and proved to be robust to glucolipotoxicity. Electrical waves revealed synchronized electrical activity and their propagation through the islet was slowed down by glucolipotoxicity. The N-methyl-d-aspartate receptor did not influence islet synchronization since modulation of the receptor only affected electrical spikes. The technique is a useful tool for exploring the pancreatic islet network in health and disease.
Authors
- Jennifer K. Briggs (ORCID: https://orcid.org/0000-0002-8737-2215)
- Jan David Hüwel (ORCID: https://orcid.org/0000-0003-4133-6232)
- Richard K.P. Benninger (ORCID: https://orcid.org/0000-0002-5063-6096)
- Christian Beecks (ORCID: https://orcid.org/0009-0000-9028-629X)
- Anne Gresch (ORCID: https://orcid.org/0000-0002-1256-2663)
- Ruben Koch
- Tim Berger (ORCID: https://orcid.org/0000-0001-6263-9123)
- Thomas Deickert
- Jana Osthues
- Martina Düfera
Institutions
- FernUniversität in Hagen (DE)
- University of Münster (DE)
- University of Colorado Anschutz Medical Campus (US)
- FH Münster University of Applied Sciences (DE)
Publication Details
- Journal
- Diabetes
- Published
- 2024-11-25
- DOI
- https://doi.org/10.2337/db23-0870
- Citations
- 3
- Primary Topic
- Pancreatic function and diabetes
- Type
- article
- Field-Weighted Citation Impact
- 1.72
Funders
- National Science Foundation
- Deutsche Forschungsgemeinschaft
- National Institute of Diabetes and Digestive and Kidney Diseases