Synthesis and characterization of the chitosan-xanthan-graphene oxide film for bone regeneration using advanced cell therapy

The present study aimed to synthesize and characterize polymer films at different graphene oxide (GO) concentrations for use in advanced cell therapy. Chitosan-xanthan (CX) films were prepared and combined with GO and then assigned to the following groups: G1 – CX; G2 – CX GO 0.5%; G3 – CX GO 1.0%; and G4 – CX GO 1.5%. The films were analyzed for their structural, mechanical, and biological properties by Raman and Fourier-Transform Infrared (FTIR) spectroscopy, Thermogravimetric Analysis (TGA), Scanning Electron Microscopy (SEM), Confocal Laser Scanning Microscopy (surface roughness measurement), Tensile Strength, and in vitro Bioactivity assay. Tensile strength and surface roughness data were analyzed by one-way analysis of variance (ANOVA), followed by Tukey’s test (α = 0.05). FTIR spectroscopy showed the presence of amide I and II bands (characteristic of chitosan) and carboxyl group (characteristic of xanthan). CO bands characteristic of GO were observed in groups containing these particles. Raman spectroscopy revealed D and G bands in the GO-containing groups. Film surface morphology exhibited a homogeneous, compact, and pore-free surface. The CX group had higher tensile strength (5.89 ± 1.62 KPa) ( p < 0.05). No statistically significant difference was observed in the other GO-containing groups ( p > 0.05). The films induced the deposition of apatite crystals on their surfaces, exhibiting activity under biomineralization conditions. Graphene oxide added into chitosan-xanthan films enhanced surface roughness and bioactivity but decreased tensile strength. The synthesized CXGO films are promising for advanced cell therapy because of their physicochemical, morphological, and mechanical properties, which seem ideal for tissue regeneration.