Isolation of Plant Growth Promoting Microbes from the Rhizosphere of Ocimum Sanctum on Okra Crop

The present study investigates the potential of plant growth-promoting rhizobacteria (PGPR) isolated from the rhizosphere of Ocimum sanctum to enhance the growth and yield of okra (Abelmoschus esculentus). A total of 10 bacterial strains were identified, comprising proteolytic and amylolytic types, with four key isolates (S1, S2, S3, and S4) selected for field trials. These isolates were applied at three critical stages of okra growth: germination, flowering, and fruiting. Significant differences were observed between treated and control plots. Plants treated with isolates S3 and S4 exhibited superior growth, with S3-treated plants producing dark green fruits with an average length of 17 cm and an overall yield of 15 kg per plant. In contrast, control plants yielded malformed, curved fruits with an average length of 10 cm and were more susceptible to pest infestations and defoliation. The study highlights the role of PGPR in enhancing crop health, productivity, and resistance to pests. The proteolytic activity of S3 and S4 isolates proved effective in improving morphological parameters such as canopy density and fruit quality while maintaining soil fertility. These findings underscore the importance of microbial inoculants as eco-friendly, sustainable alternatives to chemical fertilizers and pesticides. The successful application of these isolates supports their potential use in vegetable cultivation for improved yield and quality without compromising environmental health. In conclusion, isolates S3 and S4 demonstrate remarkable potential as plant growth promoters and pest resistance agents in okra cultivation. Their application can significantly contribute to sustainable agricultural practices and address the challenges posed by chemical inputs. Further research is recommended to explore their scalability and impact on other crops.