Development of Dembo-PCR for cattle in accordance with Japanese livestock quarantine guidelines

Detection of microorganisms from bovine using real-time PCR (Dembo-PCR) is a comprehensive detection technique that was developed to detect pathogens causing bovine diseases. In Japan, the definitive tests for monitored infectious diseases, which are defined by law, are carried out at government agencies. On the other hand, the existence of pathogens other than monitored infectious diseases are not well understood. From the perspective of livestock quarantine, it is important to make it possible to identify pathogens other than monitored infectious diseases, so we extended the existing Dembo-PCR system in this study. In particular, the number of targets other than monitored infectious disease was increased. The new version of Dembo-PCR may be useful in elucidating new pathologies associated with multiple pathogens.

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Publication Details

Journal
Journal of Veterinary Medical Science
Published
2024-11-24
DOI
https://doi.org/10.1292/jvms.24-0030
Primary Topic
Molecular Biology Techniques and Applications
Type
article
Field-Weighted Citation Impact
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article

Development of Dembo-PCR for cattle in accordance with Japanese livestock quarantine guidelines

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Journal of Veterinary Medical Science
Molecular Biology Techniques and Applications
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Development of Dembo-PCR for cattle in accordance with Japanese livestock quarantine guidelines

Yuka Nunomura, Tomoya ENOMOTO, Tsutomu Omatsu, Seiichi Kakinuma, Masashi Oguro, Hidetoshi Morita, Maydelín Frontela Noda, Makoto Nagai, Mami Oba, Ryo Imai, Natsuko Teshima, Tetsuya Mizutani, Kazuki Ito, Kazunari KONAKA
article en

Abstract

Detection of microorganisms from bovine using real-time PCR (Dembo-PCR) is a comprehensive detection technique that was developed to detect pathogens causing bovine diseases. In Japan, the definitive tests for monitored infectious diseases, which are defined by law, are carried out at government agencies. On the other hand, the existence of pathogens other than monitored infectious diseases are not well understood. From the perspective of livestock quarantine, it is important to make it possible to identify pathogens other than monitored infectious diseases, so we extended the existing Dembo-PCR system in this study. In particular, the number of targets other than monitored infectious disease was increased. The new version of Dembo-PCR may be useful in elucidating new pathologies associated with multiple pathogens.

Journal of Veterinary Medical ScienceVol. 87(1)
Saitama Prefecture (JP), Veterinary Medical Center (JP), Tochigi Medical Center (JP), Fujita Animal Hospital (JP), Kanagawa Prefecture Medical Association (JP), Azabu University (JP), Saitama University (JP), Shimadzu (Japan) (JP), Tokyo University of Agriculture and Technology (JP)
Openalex Percentile: Top 81%
Molecular Biology Techniques and Applications
DOIOpen Access PDFOpenAlex
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