Rapid and supersensitive allele detection of Plasmodium falciparum chloroquine resistance via a Pyrococcus furiosus argonaute-triggered dual-signal biosensing platform
This study established an RPA-PfAgo platform for genotyping the key mutation Pfcrt-CVMNK/CVIET of CQR. This method can rapidly produce reliable results and avoid the disadvantages of nPCR with sequencing. This approach has the characteristics of a short operation time, low device dependence, and a good match to the POCT strategy, suggesting that the platform can be easily applied locally or on site.
Authors
- Yuxia Wei (ORCID: https://orcid.org/0000-0002-3390-0844)
- Huagui Wei
- Wen‐chang Lin (ORCID: https://orcid.org/0000-0003-0194-1745)
- Haibei Hu
- Junli Wang (ORCID: https://orcid.org/0000-0003-2636-7591)
- Jian Li (ORCID: https://orcid.org/0000-0001-9434-7803)
- Cheng Zhang (ORCID: https://orcid.org/0000-0002-4134-2854)
- Chunfang Wang (ORCID: https://orcid.org/0009-0004-5611-4370)
- Liying Chen (ORCID: https://orcid.org/0000-0003-0990-7648)
- Wencheng Chen (ORCID: https://orcid.org/0000-0002-2135-6802)
- Min Lin (ORCID: https://orcid.org/0009-0006-1303-7429)
- Zongyun Lin
- Daiqian Zhu
- Jiang‐Tao Chen (ORCID: https://orcid.org/0009-0003-9012-1067)
- Xue‐Yan Liang
Institutions
- Hubei University of Medicine (CN)
- Affiliated Hospital of Youjiang Medical University for Nationalities (CN)
- Huizhou Central People's Hospital (CN)
Publication Details
- Journal
- Parasites & Vectors
- Published
- 2024-11-24
- DOI
- https://doi.org/10.1186/s13071-024-06575-0
- Citations
- 5
- Primary Topic
- Malaria Research and Control
- Type
- article
- Field-Weighted Citation Impact
- 2.71