Sample loading in gel electrophoresis using adapted 3D printers
In gel electrophoresis, samples that are dispensed too high above or too low into the wells result in sub-optimal outcomes. Here, an adapted 3D printer liquid handler equipped with an optical sensor was found to attain vertical sample delivery positionings at a standard deviation over mean ratio of 0.008. This illustrated high accuracy and repeatability outcomes achieved using automation that is cost effective and low in technical knowledge demand.
Authors
- Thomas J. Hiscox (ORCID: https://orcid.org/0000-0003-2182-2874)
- Oi Wah Liew (ORCID: https://orcid.org/0000-0001-5729-2700)
- Hassan Ali Abid (ORCID: https://orcid.org/0000-0001-7270-9463)
- Tuck Wah Ng (ORCID: https://orcid.org/0000-0001-5592-1390)
- Yuki Yew
- Tao Tao (ORCID: https://orcid.org/0000-0001-9862-8178)
- Fawaz El-Dani
- Alexey Kopyl
Institutions
- King Fahd University of Petroleum and Minerals (SA)
- National University of Singapore (SG)
- National University Health System (SG)
- Monash University (AU)
Publication Details
- Journal
- Analytical Biochemistry
- Published
- 2024-11-23
- DOI
- https://doi.org/10.1016/j.ab.2024.115721
- Primary Topic
- Microfluidic and Capillary Electrophoresis Applications
- Type
- article
- Field-Weighted Citation Impact
- 0.00