Molecular Characterisation of Some Corn (Zea mays L.) Genotypes Growing in Iğdır Province

Inter-primer binding site markers based on retrotransposons or iPBS retrotransposon markers have been useful in determining genetic diversity in a large number of organisms. The CTAB technique was employed to isolate DNA, and iPBS molecular markers were employed to conduct genetic diversity studies. Based on the analyses' findings, the genotypes exhibited a significant degree of genetic diversity, with a 100% polymorphic locus percentage. A total of 154 polymorphic bands were generated as a consequence of molecular identification experiments conducted with 12 IPBS markers. The typical polymorphism rate was determined to be 100%. Additionally, the average polymorphism (PIC) value, which quantifies the gene diversity of all markers examined in the study, was 0.228, and the average H value was 0.274. The genotypes 1 and 14 exhibited the lowest similarity ratio, with a coefficient value of 0.1600, when the DICE similarity coefficients were compared. These results were derived by comparing the DICE similarity coefficients of the samples. The analysis determined that the greatest similarity value between genotypes 4 and 3 was 0.6747. Maize genotypes are classified into four distinct subpopulations. Genotypes can be developed for reproductive research in order to demonstrate the molecular diversity of a population. It was determined that IPBS molecular markers are appropriate genetic instruments for genetic and phylogenetic analyses in maize varieties. This conclusion was attained as a consequence of the aforementioned. The data that is gathered will serve as a scientific foundation and a valuable contribution to the field of maize genetics in the future.