Factorial Analysis on the Production of Biofertilizer with High Nitrogen Content Using Mushroom Waste

This study investigated factors influencing the production of high nitrogen (N) biofertilizer from mushroom waste, examining five factors: mushroom waste (MW) content (30% to 70%), Bokashi fermentation time (5 to 10 days), agitation (Yes or No), mushroom waste size (Cut or Uncut), and drying temperature (60°C to 105°C). Through Two-Level Factorial Analysis (TLFA), the most impactful factors on N content were identified. Using a HACH Spectrophotometer as measurements devices, optimal conditions were found: 104°C drying temperature, 70% MW content, uncut mushroom waste, 10 days of Bokashi fermentation with agitation, resulting in 1624 mg/L N content. Future research is to assess the long-term stability and efficacy of the biofertilizer in various soil types and climates and conduct scale-up studies to evaluate industrial production feasibility and efficiency. Ongoing research could transform these alternative materials into valuable natural products, promoting sustainable practices and reducing overall waste.