Fine mapping of a major QTL on chromosome A05 conferring pod size in peanut

Pod size is a key agronomic trait that influences peanut yield greatly. However, our understanding of the mechanisms underlying pod size is limited. In this study, we employed a segregating population derived from a cross between the small-pod line ND_S and the large-pod line ND_L to map quantitative trait loci (QTL) associated with pod size. Initial mapping performed using bulk segregant analysis revealed a candidate interval on chromosome A05 referred to as qPSW05 . We refined this interval to a 256.9 kb genomic region using newly developed molecular markers. Through sequence and expression analyses, we identified the candidate gene AhXE45GC , which encodes an AN1 zinc finger protein. We discovered a 33-bp insertion in the intron of AhXE45GC in ND_S. Accessions that lack this insertion, such as ND_L, had significantly larger pods than those with the insertion, including ND_S. To facilitate marker-assisted selection for peanut pod size, we developed a molecular marker associated with this polymorphism. This marker could provide a valuable genetic resource for breeding high-yielding peanut varieties.

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Publication Details

Journal
The Crop Journal
Published
2024-11-23
DOI
https://doi.org/10.1016/j.cj.2024.10.009
Citations
2
Primary Topic
Peanut Plant Research Studies
Type
article
Field-Weighted Citation Impact
1.20
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article

Fine mapping of a major QTL on chromosome A05 conferring pod size in peanut

Di Cao, Ding Qiu, Sasa Hu, Kunkun Zhao et al.
2 citations
The Crop Journal
Peanut Plant Research Studies
1.20
article

Fine mapping of a major QTL on chromosome A05 conferring pod size in peanut

Di Cao, Ding Qiu, Sasa Hu, Kunkun Zhao, Zhong‐Feng Li, Xiongming Du, Xingli Ma, Fangping Gong, Zenghui Cao, Lin Zhang, Jingjing Zhang, Qian Ma, Kai Xuan Zhao, Dongmei Yin, Yaoyao Li, Xing‐Guo Zhang, Yi Fan, Rui Ren
article en
2 citations

Abstract

Pod size is a key agronomic trait that influences peanut yield greatly. However, our understanding of the mechanisms underlying pod size is limited. In this study, we employed a segregating population derived from a cross between the small-pod line ND_S and the large-pod line ND_L to map quantitative trait loci (QTL) associated with pod size. Initial mapping performed using bulk segregant analysis revealed a candidate interval on chromosome A05 referred to as qPSW05 . We refined this interval to a 256.9 kb genomic region using newly developed molecular markers. Through sequence and expression analyses, we identified the candidate gene AhXE45GC , which encodes an AN1 zinc finger protein. We discovered a 33-bp insertion in the intron of AhXE45GC in ND_S. Accessions that lack this insertion, such as ND_L, had significantly larger pods than those with the insertion, including ND_S. To facilitate marker-assisted selection for peanut pod size, we developed a molecular marker associated with this polymorphism. This marker could provide a valuable genetic resource for breeding high-yielding peanut varieties.

The Crop JournalVol. 12(6)
Henan Agricultural University (CN)
Zero hunger
Openalex Percentile: Top 16%
Peanut Plant Research Studies
1.20
DOIOpen Access PDFOpenAlex
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