Screening the Global Health Priority Box against Plasmodium berghei liver stage parasites using an inexpensive luciferase detection protocol
This method is suitable for high-throughput screening at a cost nearly 20-fold less than using commercial luciferase detection kits, thereby enabling larger liver stage anti-malarial screens and hit optimization make-test cycles. Further optimization of the hits detected using this protocol is ongoing.
Authors
- Steven P. Maher (ORCID: https://orcid.org/0000-0002-9560-5656)
- Anne Elliot
- Ashutosh K. Pathak
- Rafael Maciel‐de‐Freitas (ORCID: https://orcid.org/0000-0002-2198-6492)
- Dennis E. Kyle (ORCID: https://orcid.org/0000-0002-0238-965X)
- Anthony A. Ruberto (ORCID: https://orcid.org/0000-0002-3215-9484)
- Ritu Sharma (ORCID: https://orcid.org/0009-0009-0074-5612)
- Caitlin Cooper (ORCID: https://orcid.org/0000-0002-9994-0291)
- Olivia McWhorter
- Gia-Bao Nguyen
Institutions
- University of Georgia (US)
Publication Details
- Journal
- Malaria Journal
- Published
- 2024-11-23
- DOI
- https://doi.org/10.1186/s12936-024-05155-y
- Citations
- 3
- Primary Topic
- Malaria Research and Control
- Type
- article
- Field-Weighted Citation Impact
- 1.63
Funders
- Georgia Research Alliance
- Medicines for Malaria Venture
- Global Health Innovative Technology Fund
- National Institutes of Health
- National Institute of Allergy and Infectious Diseases