Invitro Propagation of Pomegranate (Punica Granatum L.) Cv. Wonderful Cultivar

The conventional method of propagation of pomegranate is time-consuming and tiresome. It does not ensure disease-free and healthy plants. The Present investigation aimed to optimize the conditions of culture medium, shoot proliferation, and rooting for mass production of pomegranate trees (Punica granatum L.) cv. ʻWonderful Cultivarʼ. For sterilization of axillary bud explants and shoot apexes of wonderful pomegranate treatment involving HgCl2 (0.1 %) for 3 min gave better sterilization. An efficient in vitro propagation for wonderful pomegranate using shoot tip and axillary bud explants is described. The best media for the establishment was observed on DKW medium for the first three weeks. After that, explants were subcultured on a WPM medium for proliferation and elongation. ½MS and full MS were used for rooting. The maximum number of shoots (3.9) per explant and shoot length (1.5 to 2.95 cm) were obtained on WPM medium supplemented with 0.8 mg/l B A P + 0.01 mg/L IBA in the medium. The highest rooting number was recorded on ½MS medium containing IBA 1mg/l—(1.4) roots per explant. A significantly higher number of shoots (2.5) and maximum length (2.95cm) of the shoot were recorded in 3 percent sucrose, (3000 lux) light intensity, and pH 5.8. Rooted plantlets were adopted and transferred to the soil successfully.