Quantitative phase imaging through scattering layer by lensless digital holographic microscopy

The label-free computational techniques with phase imaging capability unfolds the domain of quantitative phase imaging (QPI) to a broad range of microscopy applications. However, the presence of scattering layers in the propagation path makes the conventional QPI approaches susceptible due to the light scattering effects in the medium. Owing to this phase imaging challenge through scattering layers, we propose and experimentally demonstrate a technique for the quantitative imaging through scattering layers by integrating the lens-less digital holographic microscopy with speckle correlation approach. The proposed technique utilizes a holographic compact microscopy scheme for the recovery of phase information of the object located at any arbitrary planes behind the scattering medium by utilizing a correlation hologram and an in-line hologram. The QPI capability of the developed system is theoretically introduced and experimentally validated by imaging various standard and unknown phase samples.