3D dual-modality refractive index and fluorescence microscopy based on electrically tunable lens

Label-free microscopy that does not require the staining of weakly absorbing samples circumvent the adverse effects of exogenous dyes on the biological sample, and thus have received much attention in recent years. Among them, non-interferometric optical diffraction tomography microscopy has become a hot topic in the direction of label-free three-dimensional microscopy due to its system simplicity, ease of integration and independence from scattering noise. We recently designed a refractive index and fluorescence dual-modality microscopy system based on the transport of intensity diffraction tomography microscopy, which solves the absorption and phase information of the sample separately with the help of two illuminating apertures, and optimizes the imaging speed by applying an electrically tunable lens.