Development and Validation of HPLC Assay Method for Determination of Marbofloxacin in Veterinary Formulation Injectables

A novel gradient reverse-phase chromatographic analytical method was developed and validated for the quantitative determination of Marbofloxacin in veterinary formulation injectables using HPLC-DAD.Marbofloxacin was eluted in gradient mode using the mobile phase of 0.1% formic acid in 10mM ammonium formate in water (mobile phase-A) and 0.1% formic acid in methanol (Mobile Phase-B) at a flow-rate of 1.5mL/min on Inertsil ODS-3, C184.6mm,5m, particle size column.The present method was found to be sensitive and selective at very low levels of linearity range 1.986 to 14.892 g/mL with a correlation coefficient of 0.999.The mean accuracy expressed as recovery of the method was 101.4%.The precision (%RSD) for intra & inter day was < 2%.The method has adequate sensitivity with detection and quantitation of 1.0 g/mL.The method was strictly validated according to the ICH and AOAC guidelines.Based on the presented results, the proposed method was considered more precise, accurate with adequate sensitivity and robust.Acquired results demonstrated that proposed method can be applied for quantitative analysis of Marbofloxacin in veterinary formulation injections.