Protein crystallization and structure determination at room temperature in the <scp>C</scp>rystal<scp>C</scp>hip
The production of high-quality crystals is a key step in crystallography in general, but control of crystallization conditions is even more crucial in serial crystallography, which requires sets of crystals homogeneous in size and diffraction properties. This protocol describes the implementation of a simple and user-friendly microfluidic device that allows both the production of crystals by the counter-diffusion method and their in situ analysis by serial crystallography. As an illustration, the whole procedure is used to determine the crystal structure of three proteins from data collected at room temperature at a synchrotron radiation source.
Authors
- Petr Pachl (ORCID: https://orcid.org/0000-0001-9215-9817)
- Christophe Ritzenthaler (ORCID: https://orcid.org/0000-0001-7121-2359)
- Sylvain Engilberge (ORCID: https://orcid.org/0000-0001-8680-6790)
- Pavlína Řezáčová (ORCID: https://orcid.org/0000-0001-9626-346X)
- C. Sauter (ORCID: https://orcid.org/0000-0002-8766-287X)
- Léna Coudray
- Hélène Scheer (ORCID: https://orcid.org/0000-0001-8052-1129)
- Romain Vincent
- Léa Nilles
- Adéla Fejfarová
Institutions
- Centre National de la Recherche Scientifique (FR)
- Commissariat à l'Énergie Atomique et aux Énergies Alternatives (FR)
- CEA Grenoble (FR)
- Institut de Biologie Moléculaire des Plantes (FR)
- Architecture et Réactivité de l'arN (FR)
- Czech Academy of Sciences, Institute of Organic Chemistry and Biochemistry (CZ)
- Institut de Biologie Structurale (FR)
- Institut de Biologie Moléculaire et Cellulaire (FR)
- Université de Strasbourg (FR)
- Université Grenoble Alpes (FR)
Publication Details
- Journal
- FEBS Open Bio
- Published
- 2024-11-21
- DOI
- https://doi.org/10.1002/2211-5463.13932
- Citations
- 2
- Primary Topic
- Enzyme Structure and Function
- Type
- article
- Field-Weighted Citation Impact
- 0.23
Funders
- European Commission
- Agence Nationale de la Recherche
- European Synchrotron Radiation Facility
- Ministère de l'Europe et des Affaires Étrangères
- Université de Strasbourg
- Centre National de la Recherche Scientifique
- Institut des sciences biologiques