Enhanced extracellular production of Coprinopsis cinerea laccase Lcc9 in Aspergillus niger by gene expression cassette and bioprocess optimization
To our knowledge, this study is the first to report the recombinant extracellular production of the C. cinerea laccase Lcc9 in A. niger and to use SP<sub>CAT</sub> in the A. niger expression system. The results of this study will help accelerate the industrial application of Lcc9. Moreover, the strategy used in this work provides methodological guidance for increasing other exogenous protein yields in A. niger.
Authors
- Zemin Fang (ORCID: https://orcid.org/0000-0002-9853-5470)
- Xiaozhuang Liu
- Dongbang Yao
- Juanjuan Liu (ORCID: https://orcid.org/0000-0002-1062-4488)
- Yazhong Xiao (ORCID: https://orcid.org/0000-0003-2664-742X)
- Hui Wang (ORCID: https://orcid.org/0000-0002-3558-0055)
Institutions
- University of Science and Technology of China (CN)
- Anhui University (CN)
Publication Details
- Journal
- BMC Biotechnology
- Published
- 2024-11-22
- DOI
- https://doi.org/10.1186/s12896-024-00924-8
- Citations
- 1
- Primary Topic
- Enzyme-mediated dye degradation
- Type
- article
- Field-Weighted Citation Impact
- 0.60
Funders
- National Natural Science Foundation of China
- Anhui University
- Hefei University
- Hefei University of Technology
- National Key Research and Development Program of China