A Selective Culture Medium for Screening Aztreonam-Avibactam Resistance in Enterobacterales and <i>Pseudomonas aeruginosa</i>
Aztreonam/avibactam (ATM/AVI) has been recently approved drug for clinical use in the European Union. The aim of this study was to develop and evaluate a novel selective medium for the isolation of ATM/AVI-resistant strains (Super ATM/AVI selective medium) to help to control their spread. Minimum inhibitory concentrations of ATM/AVI were determined using the broth microdilution method for 77 Gram-negative isolates, including 62 Enterobacterales and 15 <i>Pseudomonas aeruginosa</i>. The Super ATM/AVI selective medium was elaborated using optimal final concentrations of ATM at 5 mg/L and AVI at 4 mg/L, being supplemented with amphotericin B and vancomycin to prevent growth of yeasts and Gram-positive bacteria and with ZnSO<sub>4</sub> to optimize the expression of metallo-β-lactamase producers. Super ATM/AVI showed high sensitivity (94.6%) and specificity (100%) at a detection limit of 10<sup>3</sup> CFU/mL.
Authors
- Elisa Cordero (ORCID: https://orcid.org/0000-0001-7766-7266)
- Patrice Nordmann (ORCID: https://orcid.org/0000-0002-1343-1622)
- Soraya Herrera-Espejo (ORCID: https://orcid.org/0000-0001-8019-0905)
- María Eugenia Pachón-Ibáñez (ORCID: https://orcid.org/0000-0001-7969-8162)
- Laurent Poirel (ORCID: https://orcid.org/0000-0001-5160-5286)
- Maxime Bouvier (ORCID: https://orcid.org/0000-0003-0094-5373)
Institutions
- University of Fribourg (CH)
- Instituto de Salud Carlos III (ES)
- Centro de Investigación Biomédica en Red (ES)
- Instituto de Biomedicina de Sevilla (ES)
- Hospital Universitario Virgen del Rocío (ES)
- Universidad de Sevilla (ES)
Publication Details
- Journal
- Microbial Drug Resistance
- Published
- 2024-11-22
- DOI
- https://doi.org/10.1089/mdr.2024.0150
- Primary Topic
- Antibiotic Resistance in Bacteria
- Type
- article
- Field-Weighted Citation Impact
- 0.00