Natural variation in <i>GhROPGEF5</i> contributes to longer and stronger cotton fibers

Length and strength are key parameters impacting the quality of textiles that can be produced from cotton fibers, and therefore are important considerations in cotton breeding. Through map-based cloning and function analysis, we demonstrated that GhROPGEF5, encoding a ROP guanine nucleotide exchange factor, was the gene controlling fiber length and strength at qFS_A10.1. Evolutionary analysis revealed that a base deletion in the third exon of GhROPGEF5 resulting in superior fiber length and strength was a rare mutation occurring in a tiny percentage of Upland cottons, with reduced fiber yield hindering its spread. GhROPGEF5 interacted with and activated GhROP10. Knockout or mutation of GhROPGEF5 resulted a loss of the ability to activate GhROP10. Knockout of GhROPGEF5 or GhROP10 affected the expression of many downstream genes associated with fiber elongation and secondary wall deposition, prolonged fiber elongation and delayed secondary wall deposition, producing denser fiber helices and increasing fiber length and strength. These results revealed new molecular aspects of fiber development and revealed a rare favorable allele for improving fiber quality in cotton breeding.