Engineering Saccharomyces cerevisiae for the production of natural osmolyte glucosyl glycerol from sucrose and glycerol through Ccw12-based surface display of sucrose phosphorylase
The here-described S. cerevisiae strain, displaying C-terminally anchored sucrose phosphorylase on its cell surface, is the first eukaryotic microbial cell factory capable of a one-step αGG production from the readily available substrates sucrose and glycerol.
Authors
- Anita Slavica (ORCID: https://orcid.org/0000-0003-3891-787X)
- Nenad Marđetko (ORCID: https://orcid.org/0000-0002-3857-9818)
- Bojan Žunar (ORCID: https://orcid.org/0000-0002-6855-7222)
- Renata Teparić (ORCID: https://orcid.org/0000-0002-3454-700X)
- Antonija Trontel (ORCID: https://orcid.org/0000-0003-2307-5585)
- Tea Martinić Cezar (ORCID: https://orcid.org/0009-0004-7814-3156)
- Antonia Paić (ORCID: https://orcid.org/0009-0007-6856-9710)
Institutions
- University of Zagreb (HR)
Publication Details
- Journal
- Journal of Biological Engineering
- Published
- 2024-11-22
- DOI
- https://doi.org/10.1186/s13036-024-00468-7
- Citations
- 3
- Primary Topic
- Enzyme Catalysis and Immobilization
- Type
- article
- Field-Weighted Citation Impact
- 0.70
Funders
- Ministarstvo znanosti i obrazovanja
- European Commission
- Hrvatska Zaklada za Znanost