GAFchromic EBT film lateral resolution and contrast reproduction in the UV-blue range

The sensitivity of radiochromic films to UV-blue light is increasingly considered for light dosimetry purposes, owing to their bidimensional detection capabilities and ease of use. While film response to radiation intensity has been widely investigated by commercial scanners, spatial resolution studies remain scarce, especially for small field-of-view applications. These are of growing interest due to the antimicrobial or photo-bio-stimulating effects of UV-blue light sources in in vitro, ex vivo and in vivo models, where precise knowledge of irradiation conditions with adequate spatial resolution is crucial. In this study, we report the spatial lateral resolution and contrast reproduction of GAFchromic EBT2 and EBT3 models. Upon film irradiation by a 405 nm laser source or 365 nm LED, a confocal microscope setup was employed to read the film response at 405, 470, 488, 532 and 570 nm wavelengths, with radiant exposure of 10-70 J/cm<sup>2</sup>. The measured lateral resolution ranged from 8 to 33 μm. The film capability to reproduce contrast across various spatial frequencies (4-14 lines/mm) was evaluated using modulation transfer function analysis with irradiation performed at 365 nm and 405 nm, revealing a pronounced dependency on both radiant exposure and reading wavelength. These results confirm the film capacity to detect and resolve light intensity variability with a ~ 10 μm resolution, with notable applications in micro-beam profiling and light dosimetry.

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Publication Details

Journal
Scientific Reports
Published
2024-11-22
DOI
https://doi.org/10.1038/s41598-024-78100-3
Citations
2
Primary Topic
Advanced Radiotherapy Techniques
Type
article
Field-Weighted Citation Impact
1.22

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article

GAFchromic EBT film lateral resolution and contrast reproduction in the UV-blue range

Giacomo Insero, Tommaso Mello, G. Romano, Francesco Garzella et al.
2 citations
Scientific Reports
Advanced Radiotherapy Techniques
1.22
article

GAFchromic EBT film lateral resolution and contrast reproduction in the UV-blue range

Giacomo Insero, Tommaso Mello, G. Romano, Francesco Garzella, Antonella Battisti, Franco Fusi, Antonella Sgarbossa
article en
2 citations

Abstract

The sensitivity of radiochromic films to UV-blue light is increasingly considered for light dosimetry purposes, owing to their bidimensional detection capabilities and ease of use. While film response to radiation intensity has been widely investigated by commercial scanners, spatial resolution studies remain scarce, especially for small field-of-view applications. These are of growing interest due to the antimicrobial or photo-bio-stimulating effects of UV-blue light sources in in vitro, ex vivo and in vivo models, where precise knowledge of irradiation conditions with adequate spatial resolution is crucial. In this study, we report the spatial lateral resolution and contrast reproduction of GAFchromic EBT2 and EBT3 models. Upon film irradiation by a 405 nm laser source or 365 nm LED, a confocal microscope setup was employed to read the film response at 405, 470, 488, 532 and 570 nm wavelengths, with radiant exposure of 10-70 J/cm<sup>2</sup>. The measured lateral resolution ranged from 8 to 33 μm. The film capability to reproduce contrast across various spatial frequencies (4-14 lines/mm) was evaluated using modulation transfer function analysis with irradiation performed at 365 nm and 405 nm, revealing a pronounced dependency on both radiant exposure and reading wavelength. These results confirm the film capacity to detect and resolve light intensity variability with a ~ 10 μm resolution, with notable applications in micro-beam profiling and light dosimetry.

Scientific ReportsVol. 14(1)
University of Pisa (IT), National Enterprise for NanoScience and NanoTechnology (IT), National Research Council (IT), University of Florence (IT)
European Commission, Regione Toscana
Openalex Percentile: Top 20%
Advanced Radiotherapy Techniques
1.22
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