Streamlined miRNA loading of surface protein-specific extracellular vesicle subpopulations through electroporation
Our streamlined method integrates magnetic bead-based selection with electroporation, enabling rapid and efficient loading of miRNA into CD81<sup>+</sup> EVs. This approach not only achieves comparable miRNA loading efficiency to conventional bulk electroporation methods but also concentrates CD81<sup>+</sup> EVs and allows for simple electroporation parameter adjustment, promising advancements in therapeutic RNA delivery systems with enhanced specificity and reduced toxicity.
Authors
- Sung‐Eun Choi (ORCID: https://orcid.org/0000-0001-7534-7824)
- Corinna Torabi (ORCID: https://orcid.org/0009-0001-0735-7972)
- Soojung Hur (ORCID: https://orcid.org/0000-0002-4725-5540)
- Michael E. Paulaitis (ORCID: https://orcid.org/0000-0002-9628-1091)
- Thomas R. Pisanic (ORCID: https://orcid.org/0000-0001-5796-0836)
Institutions
- Johns Hopkins University (US)
- Johns Hopkins Medicine (US)
- Development Agency of Serbia (RS)
- Sidney Kimmel Comprehensive Cancer Center (US)
- University of Baltimore (US)
Publication Details
- Journal
- BioMedical Engineering OnLine
- Published
- 2024-11-21
- DOI
- https://doi.org/10.1186/s12938-024-01311-2
- Citations
- 11
- Primary Topic
- Extracellular vesicles in disease
- Type
- article
- Field-Weighted Citation Impact
- 2.57
Funders
- National Science Foundation
- Hartwell Foundation
- Johns Hopkins University
- National Institutes of Health
- Weill Cornell Medical College