Immunoinformatic design of a multivalent vaccine against Brucella abortus and its evaluation in a murine model using a DNA prime-protein boost strategy

Although our assays did not demonstrate the induction of a substantial protective response against <i>B. abortus</i>, this construct was capable of inducing immunogenicity. This study highlights the utility of <i>in silico</i> design for predicting and optimizing candidate vaccines and underscores the potential of using strategies such as prime boost, which incorporate antigens of different biological nature to modulate the immune response, while balancing parameters such as stability of the antigens and the cost of production.