Identification of NECTIN1 as a novel restriction factor for flavivirus infection
NECTIN1, also known as CD111 or PVRL1, has been recognized as the primary receptor for several alpha herpesviruses, including herpes simplex virus (HSV), pseudorabies virus (PRV), and bovine herpesvirus 1 (BHV-1). However, our study revealed a novel role for NECTIN1 in the virus life cycle by influencing BVDV infection. Contrary to its role as a receptor for alpha herpesviruses, NECTIN1 acts as a restriction factor for BVDV by inhibiting viral attachment via competition with CD46 for binding to the domain DD of BVDV E2. We further revealed that the replication of members of the <i>Flaviviridae</i> family was inhibited by NECTIN1, while the replication of other RNA viruses did not significantly differ. Our results demonstrate that NECTIN1 is a novel factor restricting <i>Flaviviridae</i> family virus replication and highlight the complexity of virus-host interactions and the multifaceted nature of host factors involved in viral infection.
Authors
- Quanhai Pang (ORCID: https://orcid.org/0000-0003-4436-4765)
- Zhengchen Jiang (ORCID: https://orcid.org/0000-0003-4765-8830)
- Chao Sun (ORCID: https://orcid.org/0000-0002-0577-0130)
- Lijing Wo
- Menghang Wang (ORCID: https://orcid.org/0000-0002-0776-5917)
- Haiqiao Bian
- Yan-Dong Tang (ORCID: https://orcid.org/0000-0001-7025-1364)
- Jing Wang (ORCID: https://orcid.org/0000-0001-9846-7263)
- Yuanmao Zhu
- Yongfeng Li (ORCID: https://orcid.org/0000-0002-9725-0692)
- Xin Yin (ORCID: https://orcid.org/0000-0003-2357-6718)
- Fei Xue (ORCID: https://orcid.org/0000-0002-0099-2340)
- Ying Zhang (ORCID: https://orcid.org/0000-0003-1020-101X)
- Ming Gao (ORCID: https://orcid.org/0009-0003-5338-3068)
- Yajun Guo (ORCID: https://orcid.org/0000-0002-2603-5469)
- Chaonan Wang (ORCID: https://orcid.org/0000-0002-9021-0735)
- Shuhui Qi
Institutions
- Shanxi Agricultural University (CN)
- University of Liège (BE)
- Gembloux Agro-Bio Tech (BE)
- Chinese Academy of Agricultural Sciences (CN)
- Harbin Veterinary Research Institute (CN)
Publication Details
- Journal
- mBio
- Published
- 2024-11-21
- DOI
- https://doi.org/10.1128/mbio.02708-24
- Citations
- 3
- Primary Topic
- Vector-Borne Animal Diseases
- Type
- article
- Field-Weighted Citation Impact
- 3.09
Funders
- National Natural Science Foundation of China
- Natural Science Foundation of Heilongjiang Province
- Yangzhou University
- Heilongjiang Provincial Science and Technology Department
- Science Fund for Distinguished Young Scholars of Heilongjiang Province