Discovery of two new long chain fatty acid esters of flavonol glycoside from Pollen Typhae Carbonisatus and their protective effects on HBMECs

ABATRACTTwo new compounds, namely, isorhamnenin-3-<i>O</i>-neohesperidin-6''-linoleic acid ester (<b>1</b>) and isorhamnenin-3-<i>O</i>-neohesperidin-6''-palmitate (<b>2</b>), along with 17 known compounds, were isolated from Pollen Typhae Carbonisatus (PTC). Their structures were elucidated on the basis of one-dimensional (1D)-, two-dimensional (2D)-nuclear magnetic resonance (NMR), and high-resolution electrospray ionization mass spectrometry (HRESIMS) spectroscopic data analyses. The two new compounds (<b>1</b> and <b>2</b>) exhibited a protective effect in a dose-dependent manner and promoted tube generation in the oxygen-glucose deprivation/reoxygenation (OGD/R)-induced human brain microvascular endothelial cells (HBMECs).