Maleic Acid-Butanol Pretreatment to Enhance Cellulose Accessibility for Enzymatic Hydrolysis and Ethanol Production from Oil Palm Empty Fruit Bunch

Pretreatment of lignocellulosic biomass is crucial yet challenging for sustainable energy production. This study focuses on enhancing enzymatic accessibility of cellulose in oil palm empty fruit bunches by optimizing pretreatment parameters to improve glucose and ethanol yields while reducing fermentation inhibitors. It evaluates the impact of maleic acid concentrations on biorefinery processes. High maleic acid concentrations (>25% w/w) may allow reuse and offer benefits over lower concentrations, such as enhanced delignification and increased sugar yield under milder conditions. Biomass undergoes pretreatment, enzymatic saccharification, and fermentation using <i>Saccharomyces cerevisiae</i> F118. Pretreatment with 75% maleic acid (w/w) for 60 min at 180 °C effectively removes lignin and hemicellulose, increasing cellulose accessibility but results in 74.8% crystallinity, hindering saccharification. A 50% maleic acid pretreatment yielded higher glucose (77.1%). Optimal ethanol production is achieved with 1% maleic acid pretreatment. However, the ethanol yield is negatively impacted by residual maleic acid on the solid matrix.