Creation of a Synergistic Symbiotic Based on the Consortium of Lactobacilli Limosilactobacillus fermentum 3872, Ligilactobacillus salivarius 7247, and the Actigen Prebiotic for Salmonellosis Prevention in Humans and Farm Animals

Salmonella enteritidis (SE), referred to nosocomial infections, colonizes the intestines of chickens and pigs. This leads to food contamination and increased risk of pathogen transmission to humans along the food chain. Multidrug-resistant (MDR) SE strains are particularly dangerous for newborn children and young farm animals. The antibiotics traditionally used for the treatment of salmonellosis disturb the balance of the intestinal microbiota. The development and use of synergistic synbiotics is a promising preventive direction in limiting the spread of salmonella infection. Synergistic synbiotics combine prebiotics and probiotics, which thus function optimally. The role of synbiotics in the prevention of SE remains to be elucidated. The aim of this study was to create a synergistic synbiotic with bactericidal activity and anti-adhesive properties against salmonella. As a result of the conducted research, an innovative synergistic synbiotic was created. This preparation comprises a consortium of probiotic strains of lactobacilli L. fermentum 3872 + L. salivarius 7247 and the Actigen prebiotic (a fragment of the cell wall of S. cerevisiae ). In vitro experiments revealed that the consortium has a pronounced bactericidal effect on MDR SE. The culture fluid of the consortium, together with the Actigen prebiotic, is active and exhibits synergistic anti-adhesive properties against MDR SE. Further studies will apply the results obtained for developing a protocol for preclinical studies of the created symbiotic in vivo on experimental animals.