Interaction of β-amyloid Peptide with Insulin Detemir Studied by Biolayer Interferometry and Molecular Modelling

The study of β-amyloid peptide (Aβ) interactions with protein and peptide ligands is a relevant research task. Aβ complexes with drugs that are considered as promising candidates for the therapy of Alzheimer’s disease are of particular interest. This category includes a group of hypoglycemic drugs, such as insulin detemir, a long-acting insulin analogue. In this work, the method of biolayer interferometry is used to obtain the kinetic and equilibrium parameters of interaction of insulin detemir and monomeric Aβ forms with a chain length of 40 and 42 amino acids. A model of “insulin detemir–Aβ40/42” complexes is proposed. The data obtained can be used to study the mechanisms of the effect of insulin detemir on cognitive functions of patients with Alzheimer’s disease.