Evaluation of the Chemotherapeutic Potential of Medicinal Plant Mespilus germanica Fruit Extract: Cell Death Pathways and DNA Damage Mechanism

Plant extracts are a mixture of natural complex compounds containing various biological activities, including anticancer properties. The fact that they have fewer side effects than synthetic drugs has made plant extracts an important strategy in cancer treatment The purpose of this study was to explore the chemotherapeutic potential of Mespilus germanica (medlar) fruit extract. The compound content of the extract was determined by HPLC. The proliferative concentration (PRO) and the concentration inhibiting the proliferation of half of the cells (IC50) were determined by the MTT viability test. PRO and IC50 concentrations were treated to A549 lung cancer cells for 48 hours. The study groups were determined as 3 groups: control, PRO, and IC50. Total mRNA was obtained from the cells by using the Trizol Reagent-chloroform method. cDNA synthesis was performed from total mRNA. mRNA gene expression levels of programmed cell death markers were detected by RT-qPCR. For all group studies, p