Restoration of Normal Blood Coagulation by Chitosan when Modeling Hypocoagulation with Aspirin

A study of changes in blood coagulation indices in vitro at different times of incubation (5 and 30 min) of chitosan (CTS) with normal and with hypocoagulation plasma in rats was carried out. The addition of CTS to the plasma of normal animals led to an increase in its procoagulant activity regardless of the duration of the incubation. However, an increase in the time to 30 min contributed to a more significant decrease in the fibrin-depolymerization activity of the blood. The incubation of CTS with hypocoagulation plasma obtained after the administration of acetylsalicylic acid to animals (once, orally at a dose of 2 mg/kg) for both 5 and 30 min led to the activation of blood coagulation. Under these conditions, a prolongation of the incubation time caused more pronounced effects of this drug: a significant increase in the concentration of fibrinogen, ADP-dependent platelet aggregation, degree of fibrin polymerization, and a decrease in nonenzymatic fibrinolysis. At the same time, most of the studied hemostasis indices approach the values corresponding to a normal level. The results obtained in this work indicate the high efficiency of CTS as a treatment that restores normal blood coagulation and blocks the phenomena of bleeding in hypocoagulation states.