A dual-inducible control system for multistep biosynthetic pathways
This study presents the development and remaining challenges of a set of versatile genetic tools that are portable across well-established gammaproteobacterial chassis and capable of controlling the expression of multigene biosynthetic pathways. The enhanced σ<sup>70</sup> expression systems, combined with toehold switches, facilitate the biosynthesis and study of enzymes, recombinant proteins, and natural products, thus providing a valuable resource for producing a variety of compounds in microbial cell factories.
Authors
- András György (ORCID: https://orcid.org/0000-0001-8103-260X)
- Alexandra J. Cabral
- Andrés Felipe Carrillo Rincón (ORCID: https://orcid.org/0000-0001-9182-1135)
- Natalie G. Farny (ORCID: https://orcid.org/0000-0003-1143-4560)
Institutions
- Worcester Polytechnic Institute (US)
Publication Details
- Journal
- Journal of Biological Engineering
- Published
- 2024-11-20
- DOI
- https://doi.org/10.1186/s13036-024-00462-z
- Citations
- 5
- Primary Topic
- Microbial Metabolic Engineering and Bioproduction
- Type
- article
- Field-Weighted Citation Impact
- 1.17
Funders
- Worcester Polytechnic Institute
- Hebrew University of Jerusalem
- Oak Ridge National Laboratory