Efficacy of lycopene in reducing malondialdehyde (MDA) levels due to Lipopolysaccharide (LPS) exposure in Mice (Mus musculus)

Objective: Lipopolysaccharide (LPS) is a major component of the outer membrane of gram-negative bacteria and can trigger inflammatory and oxidative stress responses. This study aims to observe the efficacy of lycopene in reducing malondialdehyde (MDA) levels due to lipopolysaccharide (LPS) exposure in mice (Mus musculus). Method: This laboratory experimental study used 25 male mice aged 3 months with body weights of ± 25 g – 35 g, divided into five groups with five replicates each. K(-) was the control group: no LPS or lycopene was administered. K(+): received LPS 0.042 mg/kg without lycopene. P1: received LPS 0.042 mg/kg and lycopene 0.3 mg/kg. P2: received LPS 0.042 mg/kg and lycopene 0.6 mg/kg. P3: received LPS 0.042 mg/kg and lycopene 0.9 mg/kg. LPS was given on days 1 and 8 intraperitoneally, and lycopene was administered daily for 14 days. MDA levels were measured using the ELISA method, and data analysis was performed using ANOVA followed by Tukey's Test (p<0.05). Result: LPS exposure significantly increased MDA levels (p<0.05), and lycopene administration significantly reduced MDA levels (p<0.05) to normal levels similar to the control (-). Conclusion: LPS exposure significantly increased MDA levels, and lycopene administration was able to reduce MDA levels to normal limits.