Mechanism of cold exposure delaying wound healing in mice
Cold temperatures have been shown to slow skin wound healing. However, the specific mechanisms underlying cold-induced impairment of wound healing remain unclear. Here, we demonstrate that small extracellular vesicles derived from cold-exposed mouse plasma (CT-sEVs) decelerate re-epithelialization, increase scar width, and weaken angiogenesis. CT-sEVs are enriched with miRNAs involved in the regulation of wound healing-related biological processes. Functional assays revealed that miR-423-3p, enriched in CT-sEVs, acts as a critical mediator in cold-induced impairment of angiogenic responses and poor wound healing by inhibiting phosphatase and poly(A) binding protein cytoplasmic 1 (PABPC1). These findings indicate that cold delays wound healing via miR-423-3p in plasma-derived sEVs through the inhibition of the ERK or AKT phosphorylation pathways. Our results enhance understanding of the molecular mechanisms by which cold exposure delays soft tissue wound healing.
Authors
- Su‐Kang Shan (ORCID: https://orcid.org/0000-0003-2907-2190)
- Yun-Yun Wu
- Si-Yang He
- Fu‐Xing‐Zi Li
- Jia-Yue Duan
- Ye-Hui Li
- Changchun Li (ORCID: https://orcid.org/0000-0002-8030-8632)
- Ye-Chi Cao
- Ke-Xin Tang
- Li-Min Lei
- Xiao Lin (ORCID: https://orcid.org/0000-0002-6039-3630)
- Bei Guo (ORCID: https://orcid.org/0000-0003-3006-7209)
- Xi Chen (ORCID: https://orcid.org/0000-0001-8473-7920)
- Feng Xu (ORCID: https://orcid.org/0000-0003-0902-2858)
- Yanlin Wu (ORCID: https://orcid.org/0000-0003-4015-8972)
- Feng Wu (ORCID: https://orcid.org/0000-0002-9410-5713)
- Rongrong Cui (ORCID: https://orcid.org/0000-0002-4746-1686)
- Ming-Hui Zheng (ORCID: https://orcid.org/0000-0002-9854-3454)
- Ling‐Qing Yuan (ORCID: https://orcid.org/0000-0001-8602-7113)
- Jun-Jie Liu (ORCID: https://orcid.org/0009-0008-3173-4708)
Institutions
- Central South University (CN)
- Hunan University of Traditional Chinese Medicine (CN)
- Second Xiangya Hospital of Central South University (CN)
- Xiangya Hospital Central South University (CN)
Publication Details
- Journal
- Journal of Nanobiotechnology
- Published
- 2024-11-20
- DOI
- https://doi.org/10.1186/s12951-024-03009-y
- Citations
- 8
- Primary Topic
- Extracellular vesicles in disease
- Type
- article
- Field-Weighted Citation Impact
- 1.87
Funders
- National Natural Science Foundation of China
- Central South University
- Natural Science Foundation of Hainan Province
- Xiangya Hospital, Central South University