Minibioreactor arrays to model microbiome response to alcohol and tryptophan in the context of alcohol-associated liver disease
The intestinal microbiota (IM) plays a role in the severity of alcohol-associated liver disease. Modifying severe alcohol-associated hepatitis (AH) dysbiosis improves liver injury through tryptophan (Trp) metabolites and the aryl hydrocarbon receptor (AhR). However, Trp's effect on the IM in alcohol use disorder (AUD) patients remains unclear. Here, we used an in vitro microbiota modeling system named Minibioreactor arrays (MBRAs). Feces from AUD patients with or without AH were treated with low, normal, or high Trp concentrations, with subsequent treatment with alcohol. Microbiota composition and AhR activity were studied. We showed that microbial communities from donors were maintained in MBRAs. High and low Trp increased the abundance of pathogen Escherichia Shigella. In the absence of alcohol, Trp changed more bacteria in AUD IM compared to AH IM. Normal Trp increased the AhR activity. Overall, maintaining normal Trp levels may prevent dysbiosis in AUD or AH, pending in vivo confirmation.
Authors
- Sabrine Naimi (ORCID: https://orcid.org/0000-0001-6553-7560)
- Benoît Chassaing (ORCID: https://orcid.org/0000-0002-4285-769X)
- Wanchao Hu (ORCID: https://orcid.org/0000-0003-4678-7630)
- Dragos Ciocan (ORCID: https://orcid.org/0000-0003-0416-1584)
- Vanessa Liévin‐Le Moal (ORCID: https://orcid.org/0000-0002-1395-1740)
- Nicolas Trainel
- Anne‐Marie Cassard (ORCID: https://orcid.org/0000-0002-1813-5128)
- Gabriel Perlemuter (ORCID: https://orcid.org/0000-0001-9985-8725)
Institutions
- Centre National de la Recherche Scientifique (FR)
- Inserm (FR)
- Université Paris Cité (FR)
- Université Paris-Saclay (FR)
- Institut thématique Immunologie, inflammation, infectiologie et microbiologie (FR)
- Assistance Publique – Hôpitaux de Paris (FR)
- Fédération Hospitalo-Universitaire, Paris Center for Microbiome Medicine (FR)
- Institut Cochin (FR)
- Hôpital Antoine-Béclère (FR)
Publication Details
- Journal
- npj Biofilms and Microbiomes
- Published
- 2024-11-20
- DOI
- https://doi.org/10.1038/s41522-024-00602-9
- Citations
- 4
- Primary Topic
- Gut microbiota and health
- Type
- article
- Field-Weighted Citation Impact
- 0.94
Funders
- Fondation de l'Avenir pour la Recherche Médicale Appliquée
- European Commission
- Agence Nationale de la Recherche
- Institut National de la Santé et de la Recherche Médicale
- Fondation pour la Recherche Médicale
- China Scholarship Council
- Association Française pour l'Etude du Foie
- Université Paris-Sud
- Société Nationale Française de Gastro-Entérologie