Electrochemical Probing of Dopamine Dynamics During Poly(I:C)‐Induced Neuroinflammation

Viruses can infiltrate the central nervous system and contribute to depression, which may include alterations in dopamine (DA) metabolism triggered by immune responses though the specific mechanisms involved remain unclear. Here, an electrochemical system to realize the real-time dynamic monitoring of DA with high sensitivity is proposed and it is demonstrated that the viral simulator polyinosinic-polycytidylic acid (poly(I:C)) can inhibit the release of DA (from 5.595 to 0.137 µm) in neurons from the perspective of single cells, cell populations and even in vivo through the combination of multiscale electrodes, including single nanowires, carbon fibers (CFs) and 2D flexible electrodes. These findings are associated with the increase in reactive oxygen species (ROS) produced by microglia. At the molecular level, poly(I:C) significantly decreases the expression of α-synuclein and increases its phosphorylation level, whereas ROS inhibitors can reverse these pathological changes and salvage DA release to half the initial level (≈2.6 µM). These results suggest that viruses may indirectly inhibit DA system function through ROS produced in inflammatory responses and that antioxidant activity may be a potential therapeutic strategy.

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Publication Details

Journal
Small
Published
2024-11-20
DOI
https://doi.org/10.1002/smll.202407685
Citations
2
Primary Topic
Tryptophan and brain disorders
Type
article
Field-Weighted Citation Impact
0.31

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article

Electrochemical Probing of Dopamine Dynamics During Poly(I:C)‐Induced Neuroinflammation

Shuang Zhao, Guangchao Zang, Zhongyuan Zeng, Guixue Wang et al.
2 citations
Small
Tryptophan and brain disorders
0.31
article

Electrochemical Probing of Dopamine Dynamics During Poly(I:C)‐Induced Neuroinflammation

Shuang Zhao, Guangchao Zang, Zhongyuan Zeng, Guixue Wang, Haihan Yuan, Zexuan Meng, Jianhua Ran, Xianglong Zhao, Ying Meng, Li Li, Jiajia Chen, Tianqi Wang, Yuchan Zhang, Chenzhong Li, Qiang Zhou, Jian Wang
article en
2 citations

Abstract

Viruses can infiltrate the central nervous system and contribute to depression, which may include alterations in dopamine (DA) metabolism triggered by immune responses though the specific mechanisms involved remain unclear. Here, an electrochemical system to realize the real-time dynamic monitoring of DA with high sensitivity is proposed and it is demonstrated that the viral simulator polyinosinic-polycytidylic acid (poly(I:C)) can inhibit the release of DA (from 5.595 to 0.137 µm) in neurons from the perspective of single cells, cell populations and even in vivo through the combination of multiscale electrodes, including single nanowires, carbon fibers (CFs) and 2D flexible electrodes. These findings are associated with the increase in reactive oxygen species (ROS) produced by microglia. At the molecular level, poly(I:C) significantly decreases the expression of α-synuclein and increases its phosphorylation level, whereas ROS inhibitors can reverse these pathological changes and salvage DA release to half the initial level (≈2.6 µM). These results suggest that viruses may indirectly inhibit DA system function through ROS produced in inflammatory responses and that antioxidant activity may be a potential therapeutic strategy.

SmallVol. 21(2)
Chongqing University (CN), Harbin Institute of Technology (CN), Chinese University of Hong Kong, Shenzhen (CN), Chongqing University of Technology (CN), Chongqing Medical University (CN)
Chongqing Municipal Education Commission Foundation, National Natural Science Foundation of China, Natural Science Foundation of Chongqing, Chongqing Municipal Education Commission, Centre Scientifique et Technique du Bâtiment
Life below water
Openalex Percentile: Top 48%
Tryptophan and brain disorders
0.31
DOIOpen Access PDFOpenAlex
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