Protocol for sorting cancer stem cells using a combination of anti-CD133 antibody and lectin cyanovirin-N
CD133 is widely used as a marker to isolate cancer stem cells (CSCs). However, the structural ambiguity of N-glycan of CD133 limits its application in the isolation of CSCs. Here, we present a protocol to sort CSCs from tumor samples by combining CD133 with α-1,2-high-mannose type glycan chains. We describe steps for purifying and biotinylating cyanovirin-N (CVN) proteins and sorting tumor cells and CD133<sup>+</sup>α-1,2-Man<sup>+</sup> (α-1,2-linked mannose<sup>+</sup>) cells to provide more specific markers for CSCs. For complete details on the use and execution of this protocol, please refer to Wei et al.<sup>1</sup>.
Authors
- Zhijun Fan
- Ziwei Liang (ORCID: https://orcid.org/0009-0005-5540-4904)
- Shuting Geng
- Jianhai Jiang (ORCID: https://orcid.org/0000-0001-9195-9302)
- Jiayue Chen
- Yuerong Yang
- Yi Li (ORCID: https://orcid.org/0000-0001-5545-7663)
- Qihang Chen (ORCID: https://orcid.org/0009-0006-6955-5196)
- Yuanyan Wei
- Yu Si
Institutions
- Shanghai Medical College of Fudan University (CN)
- Fudan University (CN)
- Taiyuan University of Technology (CN)
Publication Details
- Journal
- STAR Protocols
- Published
- 2024-11-19
- DOI
- https://doi.org/10.1016/j.xpro.2024.103443
- Primary Topic
- Cancer Cells and Metastasis
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Natural Science Foundation of Shanghai
- National Natural Science Foundation of China
- China Postdoctoral Science Foundation