Development of selective ssDNA micro-probe for PD1 detection as a novel strategy for cancer imaging
Programmed death receptor 1, PD1, modulates the function of immune cells by providing inhibitory signals and constitutes the marker of immune exhaustion. Monitoring the level of PD1 promises a useful diagnostic approach in autoimmune diseases and cancer. Here we describe the development of an ssDNA aptamer-based molecular probe capable of specific recognition of human PD1 receptor. The aptamer was selected using SELEX, its sequence was further optimized, and the affinity and specificity were determined in biochemical assays. The aptamer was converted into a fluorescent probe and its potential in molecular imaging was demonstrated in a culture of human cells overexpressing PD1 and murine pancreatic organoids / immune cells mixed co-culture model. We conclude that the provided aptamers are suitable probes for imaging of PD1 expressing immune cells even in complex cellular models and may find future utility as diagnostic tools.
Authors
- Grzegorz Dubin (ORCID: https://orcid.org/0000-0002-5535-5760)
- Barbara Pucelik (ORCID: https://orcid.org/0000-0002-0235-6532)
- Małgorzata Benedyk‐Machaczka
- Barbara Chruścicka (ORCID: https://orcid.org/0000-0003-0189-3712)
- Marta Kamińska (ORCID: https://orcid.org/0000-0002-8299-7603)
- Alicja Sochaj‐Gregorczyk
- Piotr Mydel (ORCID: https://orcid.org/0000-0001-5072-9391)
- Anna Czarna (ORCID: https://orcid.org/0000-0003-1068-0671)
- Katarzyna Magiera‐Mularz (ORCID: https://orcid.org/0000-0002-4826-6380)
- Wojciech Gałan (ORCID: https://orcid.org/0000-0001-6583-0898)
- Edyta Żyła
- Marek Winiarski (ORCID: https://orcid.org/0000-0002-5143-3098)
- Stanisłąw Malicki (ORCID: https://orcid.org/0000-0001-8778-9259)
- Joanna Kozieł (ORCID: https://orcid.org/0000-0003-3436-6425)
- Jun Wang (ORCID: https://orcid.org/0000-0002-7033-5012)
Institutions
- Jagiellonian University (PL)
- Haukeland University Hospital (NO)
- Lukasiewicz Research Network - Krakow Institute of Technology (PL)
- University of Bergen (NO)
- South China University of Technology (CN)
Publication Details
- Journal
- Scientific Reports
- Published
- 2024-11-19
- DOI
- https://doi.org/10.1038/s41598-024-74891-7
- Citations
- 2
- Primary Topic
- Advanced biosensing and bioanalysis techniques
- Type
- article
- Field-Weighted Citation Impact
- 0.47
Funders
- Fundacja na rzecz Nauki Polskiej
- Narodowym Centrum Nauki
- South China University of Technology
- Norges Forskningsråd
- Narodowa Agencja Wymiany Akademickiej
- European Regional Development Fund