Ligand binding characteristics of an NAD <sup>+</sup> riboswitch revealed by FRET and biolayer interferometry
The Class II NAD<sup>+</sup> riboswitch is a bacterial RNA that binds ligands containing nicotinamide. Herein, we report a fluorescence and biolayer interferometry study of riboswitch interactions with β-NMN. The results reveal a shift in the prevalence of a pseudoknot structure in the presence of ligand and Mg<sup>2+</sup>.
Authors
- Julia R. Widom (ORCID: https://orcid.org/0000-0002-6357-2749)
- Nico E. Conoan Nieves
Institutions
- University of Oregon (US)
Publication Details
- Journal
- Chemical Communications
- Published
- 2024-11-19
- DOI
- https://doi.org/10.1039/d4cc04261a
- Citations
- 1
- Primary Topic
- RNA and protein synthesis mechanisms
- Type
- article
- Field-Weighted Citation Impact
- 0.23
Funders
- National Institute of General Medical Sciences