Engineering a vanillate-producing strain of Pseudomonas sp. NGC7 corresponding to aromatic compounds derived from the continuous catalytic alkaline oxidation of sulfite lignin
Developing methods to overcome lignin heterogeneity is essential for its use as a raw material. Consolidating continuous alkaline oxidation of lignin in a Cu(OH)<sub>2</sub>/CF-packed flow reactor and biological funneling using an engineered catabolic pathway of Pseudomonas sp. NGC7 is a promising approach to produce VA for aromatic materials synthesis. NGC7 possesses a higher adaptability to various aromatic compounds generated from the alkaline oxidation of lignin and its natural ability to grow on p-hydroxyphenyl, guaiacyl, and syringyl compounds underscores its potential as a bacterial chassis for VA production from a wide range of lignin-derived aromatic compounds.
Authors
- M. KAMADA
- Yuta Osaka
- Katsuhiko Muraki (ORCID: https://orcid.org/0000-0002-4779-6494)
- Naofumi Kamimura (ORCID: https://orcid.org/0000-0002-8792-1675)
- Chiho Sakamoto
- Tomonori Sonoki (ORCID: https://orcid.org/0000-0002-8671-2935)
- Eiji Masai (ORCID: https://orcid.org/0000-0001-9194-7483)
- Yudai Higuchi (ORCID: https://orcid.org/0000-0002-2875-1637)
- Irwan Kurnia (ORCID: https://orcid.org/0000-0003-4762-6038)
- Chieko Yasuta
- Akihiro Yoshida (ORCID: https://orcid.org/0000-0001-7224-6550)
- Atsuko Takeuchi (ORCID: https://orcid.org/0000-0001-5582-737X)
Institutions
- Hirosaki University (JP)
- Nagaoka University of Technology (JP)
- Padjadjaran University (ID)
Publication Details
- Journal
- Microbial Cell Factories
- Published
- 2024-11-19
- DOI
- https://doi.org/10.1186/s12934-024-02590-z
- Citations
- 4
- Primary Topic
- Biochemical and biochemical processes
- Type
- article
- Field-Weighted Citation Impact
- 1.55
Funders
- Hirosaki University
- Japan Society for the Promotion of Science
- Co-creation place formation support program
- JST-Mirai Program