Complex immune network and regional consistency in the human gastric mucosa revealed by high-resolution spectral cytometry
The immune cellular landscape from the gastric mucosa remains largely unknown despite its relevance in several inflammatory conditions. Human gastric biopsies were obtained from the antrum, body and incisura from 10 individuals to obtain lamina propria mononuclear cells that were further characterized by spectral cytometry. Phenotypic hierarchical analyses identified a total of 52 different immune cell subsets within the human gastric mucosa revealing that T-cells (> 60%) and NK cells (> 20%) were the main populations. Within T-cells, CD4<sup>+</sup> and CD8<sup>+</sup> were equally represented with both subsets displaying mainly a memory and effector phenotype. NK cells, on the contrary, were largely of the early phenotype. No regional differences were observed for any subsets among the 3 locations. Following unsupervised analysis, a total of 82 clusters were found. Again, no differences were observed amongst locations although a great degree of inter-individual variability was found, largely influenced by the presence of H. pylori infection and dyspepsia. We have unraveled the human gastric immune cellular subset composition and a unique interindividual immune fingerprint with no inter-regional variations.
Authors
- Aida Fiz‐López (ORCID: https://orcid.org/0000-0003-2052-7996)
- Cándido Perez-Segurado (ORCID: https://orcid.org/0000-0002-5844-745X)
- Concepción Marañón (ORCID: https://orcid.org/0000-0002-7827-6301)
- Sara Cuesta‐Sancho (ORCID: https://orcid.org/0000-0002-9890-1063)
- Elisa Arribas‐Rodríguez (ORCID: https://orcid.org/0000-0002-4470-075X)
- Alvaro Muñoz (ORCID: https://orcid.org/0000-0002-5726-0810)
- José Antonio Garrote (ORCID: https://orcid.org/0000-0003-1797-6520)
- Paloma Cal‐Sabater (ORCID: https://orcid.org/0000-0003-0267-794X)
- Eduardo Arranz (ORCID: https://orcid.org/0000-0002-6944-0894)
- Luis Ignacio Fernández-Salazar (ORCID: https://orcid.org/0000-0002-9951-7979)
- Francisco Pérez‐Cózar (ORCID: https://orcid.org/0000-0003-3348-5502)
- David Bernardo (ORCID: https://orcid.org/0000-0002-2843-6696)
- Juan H-Vázquez (ORCID: https://orcid.org/0000-0002-4805-2403)
- Ángel De Prado
- Sandra Izquierdo
- Daniel Corrales
Institutions
- Universidad de Valladolid (ES)
- Centro de Investigación Biomédica en Red (ES)
- Instituto de Biomedicina y Genética Molecular de Valladolid (ES)
- Pfizer-University of Granada-Junta de Andalucía Centre for Genomics and Oncological Research (ES)
- Hospital Clínico Universitario de Valladolid (ES)
Publication Details
- Journal
- Scientific Reports
- Published
- 2024-11-19
- DOI
- https://doi.org/10.1038/s41598-024-78908-z
- Citations
- 2
- Primary Topic
- Helicobacter pylori-related gastroenterology studies
- Type
- article
- Field-Weighted Citation Impact
- 1.15