Tracking Insulin- and Glucagon-Expressing Cells In Vitro and In Vivo Using a Double-Reporter Human Embryonic Stem Cell Line
Differentiation protocols used to generate stem cell-derived islet cells yield heterogenous cell populations. We generated a human embryonic stem cell line that reports insulin- and glucagon-expressing cells in vitro and in vivo without altering their differentiation or function. We showed some insulin- and glucagon-expressing bihormonal cells are cell-autonomously fated to become α-like cells. This reporter cell line can be used to further study and improve stem cell-derived islet differentiation and transplantation.
Authors
- Ekaterina Filatov (ORCID: https://orcid.org/0000-0003-1984-8194)
- Angela Yang (ORCID: https://orcid.org/0000-0002-9972-4046)
- Samantha Mar (ORCID: https://orcid.org/0000-0003-0215-6052)
- Francis C. Lynn (ORCID: https://orcid.org/0000-0001-9318-1063)
- Shugo Sasaki (ORCID: https://orcid.org/0000-0002-3696-7809)
- Dahai Zhang (ORCID: https://orcid.org/0000-0001-7105-1583)
- Majid Mojibian (ORCID: https://orcid.org/0000-0002-1595-9068)
- Cuilan Nian
Institutions
- University of British Columbia (CA)
- Department of Physiological Sciences (RU)
- BC Children's Hospital (CA)
Publication Details
- Journal
- Diabetes
- Published
- 2024-11-19
- DOI
- https://doi.org/10.2337/db24-0756
- Citations
- 8
- Primary Topic
- Pancreatic function and diabetes
- Type
- article
- Field-Weighted Citation Impact
- 4.58