Brief guide to immunostaining
Immunostaining is an essential biological technique that determines the localization and level of target antigen molecules using antibodies within cells or tissues. Here, we present a brief guide to immunostaining, including the principles, methods, and different types of immunostaining. This manuscript will also provide common challenges and optimization strategies. This work will be useful for researchers with basic knowledge in immunostaining.
Authors
- Jiwon Shim (ORCID: https://orcid.org/0000-0003-2409-1130)
- Gyutae Park (ORCID: https://orcid.org/0000-0003-1614-1097)
- Sieun S. Kim
- Seung‐Jae Lee (ORCID: https://orcid.org/0000-0002-6103-156X)
Institutions
- Korea Advanced Institute of Science and Technology (KR)
- Hanyang University (KR)
Publication Details
- Journal
- Molecules and Cells
- Published
- 2024-11-19
- DOI
- https://doi.org/10.1016/j.mocell.2024.100157
- Citations
- 20
- Primary Topic
- Monoclonal and Polyclonal Antibodies Research
- Type
- article
- Field-Weighted Citation Impact
- 8.09
Funders
- National Research Foundation
- National Research Foundation of Korea
- Korea Advanced Institute of Science and Technology
- Ministry of Science and ICT, South Korea