Two new <i>Tricholoma</i> species in the sect. <i>Genuina</i> from pine forests in Japan

"<i>Matsushimeji</i>" identified as <i>Tricholoma albobrunneum</i> belonging to sect. <i>Genuina</i> is an edible mushroom commonly used in Japan. This species has been suggested to include another cryptic species with common morphological characteristics and identical internal transcribed spacer (ITS) sequences of nuclear ribosomal DNA (nuc rDNA). We compared <i>T</i>. <i>albobrunneum</i> specimens sampled in Japan and Denmark, including the holotype. Phylogenetic analyses of nuc rDNA ITS and intergenic spacer 1 (IGS1) regions, and <i>tef-1a</i> and <i>rpb2</i> genes identified two phylogroups in Japanese <i>T</i>. <i>albobrunneum</i>. In addition, both Japanese phylogroups of <i>T</i>. <i>albobrunneum</i> were distinguished from European <i>T</i>. <i>albobrunneum</i>. Concatenated phylogenetic tree analysis based on these four DNA regions also distinguished two Japanese clades within <i>T</i>. <i>albobrunneum</i>. Here, we report two new species, <i>T</i>. <i>matsushimeji</i> distributed on Honshu Island under two-needle pines and <i>T</i>. <i>miyama-matsushimeji</i> on Rishiri Island under a five-needle pine from Japan.