Analysis of the embryotoxic effects of blastocyst culture medium in vitro fertilization technology

Abstract. The culture medium for blastocysts contains components that may exhibit embryotoxicity, potentially affecting the developmental process of blastocysts and leading to abnormalities in the growth and development of offspring. This paper reviews and discusses the impact of blastocyst culture medium on the development of blastocysts and later-stage embryos. The results presented in the literature indicate that blastocyst media with different compositions not only affect development during the incubation period but may also have long-term effects on post-implantation development and maturation of embryos. The paper primarily summarizes the effects of the composition and concentration of blastocyst culture medium on embryonic development, aiming to further improve the quality of in vitro culture systems and optimize the culture conditions.