CRISPR-Cas9: Revolutionizing Crop Resistance Enhancemement

Abstract. The security of food has grown to be a serious concern in recent years, due to factors such as environmental changes, pest infestations affecting crop yields and food safety. Traditional breeding methods, such as hybrid breeding, often involve long cycles, consuming substantial human and material resources and constrained by germplasm limitations, significantly hindering the rapid development of animal and plant breeding. In contrast, CRISPR-Cas9 genome editing technology allows precise modification of genetic information inanimals and plants at the molecular level, directly optimizing target traits or imparting new genetic characteristics, demonstrating more direct and efficient breeding advantages compared to traditional methods. The implementation and advancement of the CRISPR-Cas9 genome editing system to increase plant and animal resilience is the main topic of this review, which also discusses its drawbacks and potential applications.